GM Crop Database

Database Product Description

TC1507 x NK603 (DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6)

Host Organism

Zea mays (Maize)

Trait

Herbicide tolerant, glufosinate ammonium; Herbicide tolerant,glyphosate; Insect resistant, Lepidoptera.

Trait Introduction

Traditional plant breeding and selection

Proposed Use

Production for human consumption and livestock feed.

Product Developer

DOW AgroSciences LLC

Summary of Regulatory Approvals

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

Summary of Introduced Genetic Elements Expand

Code	Name	Type	Promoter, other	Terminator	Copies	Form
cry1Fa2	cry1F delta-endotoxin	IR	ubiquitin (ubi) ZM (Zea mays) promoter and the first exon and intron	3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens)	1 functional; 1-2 partial;	Altered coding sequence for optimal expression in plant cells.
pat	phosphinothricin N-acetyltransferase	HT	CaMV 35S	CaMV 35S 3' polyadenylation signal	1 functional;
CP4 epsps	5-enolpyruvyl shikimate-3-phosphate synthase	HT	P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)	A. tumefaciens nopaline synthase (nos) 3'-untranslated region	1	CP4 EPSPS gene modified for plant-preferred codons
CP4 epsps	5-enolpyruvyl shikimate-3-phosphate synthase	HT	enhanced CaMV 35S, maize HSP70 intron chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)	A. tumefaciens nopaline synthase (nos) 3'-untranslated region	1	CP4 EPSPS gene modified for plant-preferred codons

Characteristics of Zea mays (Maize) Expand

Center of Origin	Reproduction	Toxins	Allergenicity
Mesoamerican region, now Mexico and Central America	Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.	No endogenous toxins or significant levels of antinutritional factors.	Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name	Gene	Pathogenicity
Bacillus thuringiensis var. aizawai	cry1F	While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds.
Streptomyces viridochromogenes	pat	S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.
Agrobacterium tumefaciens strain CP4	CP4 epsps	Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

TC1507 X NK603 (OECD identifier: DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6) maize is an F1 hybrid resulting from the hybridization of the insect-resistant inbred maize TC1507 ( DAS-Ø15Ø7-1) with the herbicide-tolerant maize inbred NK603 (MON-ØØ6Ø3-6). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Canada and Japan.

The stacked hybrid TC1507 X NK603 expresses three novel proteins: the delta-endotoxin Cry1F which confers resistance to the European Corn Borer and other lepidopterans, the PAT protein which confers tolerance to the herbicide glufosinate ammonium, and the CP4 EPSPS protein which confers resistance to the herbicide glyphosate. The insecticidal protein is produced by the cry1F gene and PAT is produced by the pat gene, both from TC1507. The CP4 EPSPS protein is produced by the cp4 epsps gene from NK603. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parental line please refer to the individual product descriptions in the crop database for TC1507 and NK603.
The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1F protein is not an enzyme and therefore does not affect plant metabolism. Both the PAT and CP4 EPSPS have high substrate affinities: PAT for L-Phosphinothricin, the active ingredient in glufosinate ammonium, and CP4 EPSPS for phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. Since both PAT and CP4 EPSPS differ in substrates and in mode of action, these are not expected to interact within, nor affect the metabolism of the stacked hybrid.
Southern blot analysis has confirmed that cry1F, pat and cp4 epsps genes are stably inherited in the stacked hybrid. The expression levels of Cry1F, PAT and CP4 EPSPS were comparable to those of the parental lines. The stacked hybrid displayed the same degree of tolerance to the herbicides glufosinate ammonium and glyphosate, as the parental lines.
Heterosis is expected in F1 hybrids. An example of heterosis is a substantial increase in yield in the F1 hybrid compared to the parental lines. The stacked hybrid TC1507 X NK603 is therefore expected to display heterosis, as would any conventional F1 hybrid. Heterosis in this hybrid is not expected to be caused by the introduced genes, since the gene products are not expected to interact synergistically.

Links to Further Information Expand

COGEM: Commissie Genetische Modificatie

• Import and processing of insect resistant and herbicide tolerant maize 1507 x NK603
  [PDF Size: 115.65K bytes]

Comissão Técnica Nacional de Biossegurança - CTNBio (Brazil)

• Technical Opinion No. 2053/2009
  [PDF Size: 77.91K bytes]

European Commission: Community Register of GM Food and Feed

• Notification of the placing on the Community Register of DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6
  [PDF Size: 117.05K bytes]

European Food Safety Authority

• Opinion of the European Food Safety Authority on the application for the placing on the market of insect-resistant genetically modified maize 1507 x NK603 for food and feed uses from Pioneer Hi-Bred and Mycogen Seeds.
  [PDF Size: 71.23K bytes]

Japanese Biosafety Clearing House, Ministry of Environment

• Outline of the Biological Diversity Risk Assessment Report: Type 1 Use for DAS-01507-1 x MON-00603-6 insect resistant and herbicide tolerant maize
  [PDF Size: 132.94K bytes]

Secretaria de Agricultura, Ganaderia, Pesca y Alimentos: Republica Argentina

• Noticias de la SAGPyA: La SAGPyA autorizo el uso de maiz eventos acumulados 1507 x NK603
  [PDF Size: 75.17K bytes]

Turkey Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policies

• TR Official Gazette no:28152 Biosafety Board Decision No:7
  [PDF Size: 338.40K bytes]

Uruguay National Biosafety Cabinet

• Resolution No. 48 Case No 2012/7/1/1/200 (TC1507XNK603)

This record was last modified on Monday, May 2, 2016