GM Crop Database

Database Product Description

TC1507 x NK603 (DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6)
Host Organism
Zea mays (Maize)
Trait

Herbicide tolerant, glufosinate ammonium; Herbicide tolerant,glyphosate; Insect resistant, Lepidoptera.

Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
DOW AgroSciences LLC

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Argentina 2006 2006 2008
Brazil 2009 2009 2009
Canada 2006
Colombia 2009 2009 2008
European Union 2007 2007
Honduras 2016 2016
Japan 2004 2003 2003
Korea 2004 2008
Mexico 2004 2004
Philippines 2006 2006 2014
South Africa 2011 2011
Taiwan 2009
Turkey 2011
Uruguay 2012 2012 2012

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Fa2 cry1F delta-endotoxin IR ubiquitin (ubi) ZM (Zea mays) promoter and the first exon and intron 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; 1-2 partial; Altered coding sequence for optimal expression in plant cells.
pat phosphinothricin N-acetyltransferase HT CaMV 35S CaMV 35S 3' polyadenylation signal 1 functional;
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT enhanced CaMV 35S, maize HSP70 intron chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis var. aizawai cry1F While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds.
Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

TC1507 X NK603 (OECD identifier: DAS-Ø15Ø7-1 x MON-ØØ6Ø3-6) maize is an F1 hybrid resulting from the hybridization of the insect-resistant inbred maize TC1507 ( DAS-Ø15Ø7-1) with the herbicide-tolerant maize inbred NK603 (MON-ØØ6Ø3-6). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Canada and Japan.

The stacked hybrid TC1507 X NK603 expresses three novel proteins: the delta-endotoxin Cry1F which confers resistance to the European Corn Borer and other lepidopterans, the PAT protein which confers tolerance to the herbicide glufosinate ammonium, and the CP4 EPSPS protein which confers resistance to the herbicide glyphosate. The insecticidal protein is produced by the cry1F gene and PAT is produced by the pat gene, both from TC1507. The CP4 EPSPS protein is produced by the cp4 epsps gene from NK603. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parental line please refer to the individual product descriptions in the crop database for TC1507 and NK603.
The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1F protein is not an enzyme and therefore does not affect plant metabolism. Both the PAT and CP4 EPSPS have high substrate affinities: PAT for L-Phosphinothricin, the active ingredient in glufosinate ammonium, and CP4 EPSPS for phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. Since both PAT and CP4 EPSPS differ in substrates and in mode of action, these are not expected to interact within, nor affect the metabolism of the stacked hybrid.
Southern blot analysis has confirmed that cry1F, pat and cp4 epsps genes are stably inherited in the stacked hybrid. The expression levels of Cry1F, PAT and CP4 EPSPS were comparable to those of the parental lines. The stacked hybrid displayed the same degree of tolerance to the herbicides glufosinate ammonium and glyphosate, as the parental lines.
Heterosis is expected in F1 hybrids. An example of heterosis is a substantial increase in yield in the F1 hybrid compared to the parental lines. The stacked hybrid TC1507 X NK603 is therefore expected to display heterosis, as would any conventional F1 hybrid. Heterosis in this hybrid is not expected to be caused by the introduced genes, since the gene products are not expected to interact synergistically.

Links to Further Information Expand

COGEM: Commissie Genetische Modificatie Comissão Técnica Nacional de Biossegurança - CTNBio (Brazil) European Commission: Community Register of GM Food and Feed European Food Safety Authority Japanese Biosafety Clearing House, Ministry of Environment Secretaria de Agricultura, Ganaderia, Pesca y Alimentos: Republica Argentina Turkey Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policies Uruguay National Biosafety Cabinet

This record was last modified on Friday, August 11, 2017