GM Crop Database

Database Product Description

Summary of Regulatory Approvals

Introduction Expand

TC1507xMON810xMIR162xNK603 is a stacked event variety expressing three Bt proteins to confer resistance to lepidopteran insects (Cry1Ab, Cry1F, Vip3A) and two proteins (PAT and CP4 EPSPS) conferring tolerance to the herbicides glufosinate ammonium and glyphosate.

Summary of Introduced Genetic Elements Expand

Code	Name	Type	Promoter, other	Terminator	Copies	Form
CP4 epsps	5-enolpyruvyl shikimate-3-phosphate synthase	HT	P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site chloroplast transit peptide from Arabidopsis thaliana EPSPS gene (CTP2)	A. tumefaciens nopaline synthase (nos) 3'-untranslated region	1	CP4 EPSPS gene modified for plant-preferred codons
cry1Ab	Cry1Ab delta-endotoxin (Btk HD-1)	IR	Enhanced CaMV 35S, from Cauliflower mosaic virus; maize HSP70 intron	None. Lost through 3’ truncation during integration	1	Truncated
cry1Fa2	Cry1F delta-endotoxin	IR	Ubiquitin (ubi) ZM (Zea mays) promoter and the first exon and intron	3’ polyadenylation signal from ORF25 (Agrobacterium tumefaciens)	1 functional; 1-2 partial	Altered coding sequence for optimal expression in plant cells
pat	Phosphinothricin N-acetyltransferase	HT	CaMV35 (Cauliflower mosaic virus)	CaMV35 3’polyadenylation signal (Cauliflower mosaic virus)	1 functional
pmi	mannose-6-phosphate isomerase	SM	ZmUbilnt (Zea mays poly-ubiquitin gene promoter and first intron)	Agrobacterium tumefaciens nopaline synthase (nos) 3”-untranslated region	1
vip3Aa20	vegetative insecticidal protein	IR	ZmUbilnt (Zea mays polyubiquitin gene promoter and first intron)	CaMV 35S 3’ polyadenylation signal	1	Native
CP4 epsps	5-enolpyruvyl shikimate-3-phosphate synthase	HT	enhanced CaMV 35S, maize HSP70 intron chloroplast transit peptide from A. thaliana EPSPS gene (CTP2)	A. tumefaciens nopaline synthase (nos) 3'-untranslated region	1	CP4 EPSPS gene modified for plant-preferred codons

Characteristics of Zea mays (Maize) Expand

Center of Origin	Reproduction	Toxins	Allergenicity
Mesoamerican region, now Mexico and Central America	Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.	No endogenous toxins or significant levels of antinutritional factors.	Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name	Gene	Pathogenicity
Agrobacterium tumefaciens strain CP4	CP4 epsps	Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.
Bacillus thuringiensis subsp. kurstaki	cry1Ac	Although target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals or bird given up to 10 µg protein / g body wt. There are no significant mammalian toxins or allergens associated with the host organism.
Bacillus thuringiensis var. aizawai	cry1F	While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds.
Streptomyces viridochromogenes	pat	S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.
Escherichia coli (K-12)	hyg	Escherichia coli is one of a number of microorganisms which are normal inhabitants of the colons of virtually all warm-blooded mammals. E. coli belongs to the taxonomic family known asEnterobacteriaceae, which is one of the best-defined groups of bacteria. The strain E. coli K-12 is a debilitated strain which does not normally colonize the human intestine. It has also been shown to survive poorly in the environment, has a history of safe commercial use, and is not known to have adverse effects on microorganisms or plants.
Bacillus thuringiensis strain AB88	5AT	While target lepidopteran insects are susceptible to oral doses of VIP proteins, there is no evidence of toxic effects in laboratory mammals, in birds, and in non-target arthropods, including beneficial insects.
Agrobacterium tumefaciens strain CP4	CP4 epsps	Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon.

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Links to Further Information Expand

Japan, Ministry of Agriculture, Forestry, and Fisheries

• Maize resistant to Lepidoptera and tolerant to glufosinate and glyphosate herbicides
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This record was last modified on Monday, May 8, 2017