GM Crop Database

Database Product Description

Summary of Regulatory Approvals

Introduction Expand

TC1507 x MON810 x MIR162 is a stacked event variety expressing three Bt proteins to confer resistance to lepidopteran insects (Cry1Ab, Cry1F, Vip3A) and one protein (PAT protein) conferring tolerance to the herbicide glufosinate ammonium.

Summary of Introduced Genetic Elements Expand

Code	Name	Type	Promoter, other	Terminator	Copies	Form
cry1Ab	Cry1Ab delta-endotoxin (Btk HD-1)	IR	Enhanced CaMV 35S, from Cauliflower mosaic virus; maize HSP70 intron	None. Lost through 3’ truncation during integration	1	Truncated
pmi	mannose-6-phosphate isomerase	SM	ZmUbilnt (Zea mays poly-ubiquitin gene promoter and first intron)	Agrobacterium tumefaciens nopaline synthase (nos) 3”-untranslated region	1
vip3Aa20	vegetative insecticidal protein	IR	ZmUbilnt (Zea mays polyubiquitin gene promoter and first intron)	CaMV 35S 3’ polyadenylation signal	1	Native
pat	Phosphinothricin N-acetyltransferase	HT	CaMV35 (Cauliflower mosaic virus)	CaMV35 3’polyadenylation signal (Cauliflower mosaic virus)	1 functional
cry1Fa2	Cry1F delta-endotoxin	IR	Ubiquitin (ubi) ZM (Zea mays) promoter and the first exon and intron	3’ polyadenylation signal from ORF25 (Agrobacterium tumefaciens)	1 functional; 1-2 partial	Altered coding sequence for optimal expression in plant cells

Characteristics of Zea mays (Maize) Expand

Center of Origin	Reproduction	Toxins	Allergenicity
Mesoamerican region, now Mexico and Central America	Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.	No endogenous toxins or significant levels of antinutritional factors.	Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name	Gene	Pathogenicity
Bacillus thuringiensis subsp. kurstaki	cry1Ac	Although target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals or bird given up to 10 µg protein / g body wt. There are no significant mammalian toxins or allergens associated with the host organism.
Escherichia coli (K-12)	hyg	Escherichia coli is one of a number of microorganisms which are normal inhabitants of the colons of virtually all warm-blooded mammals. E. coli belongs to the taxonomic family known asEnterobacteriaceae, which is one of the best-defined groups of bacteria. The strain E. coli K-12 is a debilitated strain which does not normally colonize the human intestine. It has also been shown to survive poorly in the environment, has a history of safe commercial use, and is not known to have adverse effects on microorganisms or plants.
Bacillus thuringiensis strain AB88	5AT	While target lepidopteran insects are susceptible to oral doses of VIP proteins, there is no evidence of toxic effects in laboratory mammals, in birds, and in non-target arthropods, including beneficial insects.
Streptomyces viridochromogenes	pat	S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.
Bacillus thuringiensis var. aizawai	cry1F	While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Links to Further Information Expand

Japan, Ministry of Agriculture, Forestry and Fisheries

• Maize resistant to lepidopterous pests and tolerant to glufosinate herbicide
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This record was last modified on Monday, June 13, 2016