GM Crop Database

Database Product Description

TC1507 x DAS-59122-7 (DAS-Ø15Ø7-1 x DAS-59122-7)
Host Organism
Zea mays (Maize)
Trade Name
Herculex® XTRA
Trait

Herbicide tolerant, glufosinate ammonium; Insect resistant, Coleoptera; Insect resistant, Lepidoptera.

Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
DOW AgroSciences LLC and Pioneer Hi-Bred International Inc.

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Brazil 2013 2013 2013
Canada 2006 2006
European Union 2010 2010 View
Japan 2005 2006 2006
Korea 2006 2008
Mexico 2006 2006
Philippines 2007 2007
South Africa 2011 2011
Taiwan 2009
Turkey 2012
United States 2005

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked hybrid TC1507 X DAS-59122-7 expresses four novel proteins: the delta-endotoxin Cry1F which confers resistance to the European Corn Borer and other lepidopterans, Cry34Ab1 and Cry35Ab1, which are also delta-endotoxins in this case insecticidal to coleopterans such as the Corn Rootworm, and the PAT protein which confers tolerance to the herbicide glufosinate ammonium. The Cry1F protein is produced by the cry1F gene and PAT is produced by the pat gene, both from TC1507. The Cry34Ab1 and Cry35Ab1 insecticidal proteins are produced by the cry34Ab1 and cry35Ab1 genes from DAS-59122-7, which also contains a copy of the pat gene. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parental line please refer to the individual product descriptions in the crop database for TC1507 and DAS-59122-7.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1F, Cry34Ab1 and Cry35Ab1 proteins are not enzymes and therefore do not affect plant metabolism. The PAT enzyme has a high substrate affinity for L-Phosphinothricin, the active ingredient in glufosinate ammonium. These proteins are not expected to interact within, nor affect the metabolism of the stacked hybrid.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Fa2 cry1F delta-endotoxin IR ubiquitin (ubi) ZM (Zea mays) promoter and the first exon and intron 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; 1-2 partial; Altered coding sequence for optimal expression in plant cells.
pat phosphinothricin N-acetyltransferase HT CaMV 35S CaMV 35S 3' polyadenylation signal 1 functional;
cry34Ab1 Cry34Ab1 delta-endotoxin IR Zea mays ubiquitin gene promoter, intron and 5' UTR Solanum tuberosum proteinase inhibitor II (PINII) 1 functional Altered coding sequence for optimal expression in maize
cry35Ab1 Cry35Ab1 delta-endotoxin IR Triticum aestivum peroxidase gene root-preferred promoter Solanum tuberosum proteinase inhibitor II (PINII) 1 functional Altered coding sequence for optimal expression in maize
pat phosphinothricin N-acetyltransferase SM Cauliflower Mosaic Virus (CaMV) 35S Cauliflower Mosaic Virus (CaMV) 35S 1 functional

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis var. aizawai cry1F While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds.
Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Bacillus thuringiensis strain PS149B1 cry34Ab1 While target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals, in birds or in non-target arthropods.
Bacillus thuringiensis strain PS149B1 cry34Ab1 While target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals, in birds or in non-target arthropods.
Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon.

Abstract Collapse

TC1507 X DAS-59122-7(OECD identifier: DAS-Ø15Ø7-1 X DAS-59122-7) maize is an F1 hybrid resulting from the hybridization of the lepidopteran-resistant inbred maize TC1507 with the coleopteran-resistant maize inbred DAS-59122-7 and marketed as Herculex® XTRA. This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Japan and Korea.

The stacked hybrid TC1507 X DAS-59122-7 expresses four novel proteins: the delta-endotoxin Cry1F which confers resistance to the European Corn Borer and other lepidopterans, Cry34Ab1 and Cry35Ab1, which are also delta-endotoxins in this case insecticidal to coleopterans such as the Corn Rootworm, and the PAT protein which confers tolerance to the herbicide glufosinate ammonium. The Cry1F protein is produced by the cry1F gene and PAT is produced by the pat gene, both from TC1507. The Cry34Ab1 and Cry35Ab1 insecticidal proteins are produced by the cry34Ab1 and cry35Ab1 genes from DAS-59122-7, which also contains a copy of the pat gene. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parental line please refer to the individual product descriptions in the crop database for TC1507 and DAS-59122-7.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1F, Cry34Ab1 and Cry35Ab1 proteins are not enzymes and therefore do not affect plant metabolism. The PAT enzyme has a high substrate affinity for L-Phosphinothricin, the active ingredient in glufosinate ammonium. These proteins are not expected to interact within, nor affect the metabolism of the stacked hybrid.

Links to Further Information Expand

Brazil Ministry of Science and Technology – MCT, National Biosafety Technical Commission – CTNBio Canadian Food Inspection Agency European Food Safety Authority European Food Safety Authority (EFSA) Japanese Biosafety Clearing House, Ministry of Environment Mexican Health Secretary Federal Commission for the Protection against Sanitary Risk Philippines Department of Agriculture, Bureau of Plant Industry Turkish Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policies U S ENVIRONMENTAL PROTECTION AGENCY

This record was last modified on Tuesday, November 3, 2015