GM Crop Database

Database Product Description

T304-40xGHB119 (BCS-GHØØ4-7 x BCS-GHØØ5-8)
Host Organism
Gossypium hirsutum (Cotton)
Trade Name
TwinLink®
Trait
Glyphosate herbicide tolerance and resistance to lepidopteran insect pests.
Trait Introduction
Agrobacterium tumefaciens-mediated plant transformation.
Proposed Use

Production for fibre, livestock feed, and human consumption.

Product Developer
Bayer CropScience USA LP

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Argentina 2014
Brazil 2011 2011 2011
Canada 2011 2011 View
Korea 2013 2012
Mexico 2012
United States 2011 2011 2012

Introduction Expand

This stacked trait cotton is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked trait cotton T304-40 x GHB119 expresses the Cry1Ab, Cry2Ae and PAT proteins.  Cry1Ab and Cry2Ae are delta-endotoxins, expressed by the inserted cry1Ab and cry2Ae genes, which confer resistance to certain lepidopteran pests of cotton, such as cotton bollworm, tobacco budworm and pink bollworm.  The PAT (phosphinothricin N-acetyltransferase) protein, expressed by the bar gene, confers tolerance to glufosinate ammonium herbicides.  The traits of each parental cotton line have been stacked, through traditional breeding, to produce this stacked trait cotton line.  A full description of each parental line may be found in the crop database for T304-40 and GHB119.

The inserted genes and respective protein products have a history of safe use and have been reviewed and approved by globally by regulatory agencies.  No interactions or negative synergistic effects have been shown or are expected in the stacked trait product.  The Cry1Ab and Cry2Ae proteins are not enzymes and therefore, have no effect on plant metabolism.  The PAT protein acetylates L-phosphinothricin, a component of glufosinate-ammonium, into a non-phytotoxic metabolite (N-acetyl-L-glufosinate).

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
bar Phosphinothricin acetyltransferase HT

Cassava vein virus promoter sequence

3’ untranslated region of nopaline synthase gene

1

Coding sequence of bar gene

bar phosphinothricin acetyltransferase HT

Cauliflower mosaic virus 35S transcript

3’ untranslated region of nopaline synthase gene

1

Coding sequence of bar gene

cry1Ab Cry1Ab delta endotoxin IR

Promoter region from subterranean clover stunt virus genome segment 7

3’me1 (Flaveria bidentis)

1 plus 1 inverted incomplete copy

cry1Ab coding sequence

cry2Ae Cry2Ae delta endotoxin IR

Cauliflower mosaic virus 35S

3’35S-RB

1

cry2Ae coding sequence

Characteristics of Gossypium hirsutum (Cotton) Expand

Center of Origin Reproduction Toxins Allergenicity

Believed to originate in Meso-America (Peruvian-Ecuadorian-Bolivian region).

Generally self-pollinating, but can be cross-pollinating in the presence of suitable insect pollinators (bees). In the U.S., compatible species include G. hirsutum, G. barbadense, and G. tomentosum.

Gossypol in cottonseed meal.

Cotton is not considered to be allergenic, although there are rare, anecdotal reports of allergic reactions in the literature.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Streptomyces hygroscopicus bar S. hygroscopicus is ubiquitous in the soil and there have been no reports of adverse affects on humans, animals, or plants.
Streptomyces hygroscopicus bar S. hygroscopicus is ubiquitous in the soil and there have been no reports of adverse affects on humans, animals, or plants.
Bacillus thuringiensis subsp Berliner cry1Ab

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Bacillus thuringiensis subsp. Dakota cry2Ae

Although target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals or bird given up to 10 µg protein / g body wt. There are no significant mammalian toxins or allergens associated with the host organism.

Modification Method Expand

T304-40 x GHB119 is a conventionally-bred stacked trait product.  

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Links to Further Information Expand


This record was last modified on Friday, March 25, 2016