GM Crop Database

Database Product Description

NK603 x T25 (MON-ØØ6Ø3-6xACS-ZMØØ3-2)
Host Organism
Zea mays (Maize)
Trait
Stacked tolerance to phosphinothricin (glufosinate ammonium) and glyphosate herbicides.
Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
Monsanto Company

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Brazil 2015 2015 2015
Canada 2010
Colombia 2012 2012
European Union 2015 2015
Japan 2009 2010 2010
Korea 2011 2011
Mexico 2010 2010
Philippines 2010 2010
South Africa 2016 2016
Taiwan 2011

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
bla beta lactamase SM bacterial promoter Truncated beta-lactamase gene missing about 25 % of the gene from the 5' end; not expressed
pat phosphinothricin N-acetyltransferase HT CaMV 35S CaMV 35S poly(A) signal 3 or 1* Modified for enhanced expression; PEG mediated uptake into plant protoplasts; 3 copies in T14, 1 copy in T25
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT enhanced CaMV 35S, maize HSP70 intron chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Abstract Collapse

NK603 X T25 (OECD identifier: MON-ØØ6Ø3-6 x ACS-ZMØØ3-2) maize is an F1 hybrid resulting from the hybridization of the glyphosate herbicide-tolerant maize inbred NK603 (MON-ØØ6Ø3-6) with the glufosinate ammonium herbicide-tolerant maize inbred T25 (ACS-ZMØØ3-2). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
The stacked hybrid NK603 X T25 expresses two novel proteins: CP4 EPSPS which confers tolerance to the herbicide glyphosate, and phosphinothricin N-acetyltransferase (PAT) which confers tolerance to the herbicide glufosinate ammonium. The CP4 EPSPS protein is produced by the cp4 epsps gene from NK603, and the PAT protein by the pat gene from T25. The novel traits of each parent line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for NK603 and T25.
The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid.

Links to Further Information Expand


This record was last modified on Friday, October 28, 2016