GM Crop Database

Database Product Description

NK603 x MON810 (MON-ØØ6Ø3-6, MON-ØØ81Ø-6)
Host Organism
Zea mays (Maize)
Trait
Resistance to lepidopteran pests and glyphosate herbicide tolerance
Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
Monsanto Company

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Argentina 2005 2005 2007
Brazil 2009 2009 2009
Canada 2001
Colombia 2009
El Salvador 2009 2009
European Union 2007 2007 View
Japan 2004 2004 2004
Korea 2004
Mexico 2004 2004
Philippines 2004 2004 2005
South Africa 2004 2004 2007
Taiwan 2009
Uruguay 2011 2011 2011

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT enhanced CaMV 35S, maize HSP70 intron chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons
cry1Ab Cry1Ab delta-endotoxin (Btk HD-1) IR enhanced CaMV 35S, maize HSP70 intron None. Lost through 3' truncation during integration 1 Truncated

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Bacillus thuringiensis subsp. kurstaki EC2.4.2.19

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

NK603 X MON810 (OECD identifier: MON-ØØ6Ø3-6 x MON-ØØ81Ø-6) maize is an F1 hybrid resulting from the hybridization of the herbicide-tolerant maize inbred NK603 (MON-ØØ6Ø3-6) with the insect-resistant maize inbred MON 810 (MON-ØØ81Ø-6). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Canada and Japan.

The stacked hybrid NK603 X MON810 expresses two novel proteins: CP4 EPSPS which confers tolerance to the herbicide glyphosate, and the delta-endotoxin Cry1Ab which confers resistance to the European Corn Borer and other lepidopterans. The CP4 EPSPS protein is produced by the cp4 epsps gene from NK603, and the insecticidal protein Cry1Ab, by the cry1Ab gene from MON810. The novel traits of each parent line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for NK603 and MON810.
The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1Ab protein is not an enzyme and therefore does not affect plant metabolism. The CP4 EPSPS has high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. CP4 EPSPS and Cry1Ab are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid.
Studies were conducted to compare the resistance to lepidopterans and tolerance to glyphosate between the stacked cotton line and each parental line. There were no significant differences in the resistance to the lepidopteran pests, or in the tolerance to applications of glyphosate in the stacked line, compared to the parental lines.

Links to Further Information Expand

Comissão Técnica Nacional de Biossegurança - CTNBio (Brazil) European Commission: Community Register of GM Food and Feed European Food Safety Authority Japanese Biosafety Clearing House, Ministry of Environment Philippines Department of Agriculture, Bureau of Plant Industry Secretaria de Agricultura, Ganaderia, Pesca y Alimentos: Republica Argentina U.K. Advisory Committee on Releases to the Environment

This record was last modified on Monday, June 15, 2015