GM Crop Database

Database Product Description

MON89034 x NK603 (MON-89Ø34-3xMON-ØØØ6Ø3-6)
Host Organism
Zea mays (Maize)
Trait
Resistance to lepidopteran pests and glyphosate herbicide tolerance
Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
Monsanto Company

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Argentina 2012 2012 2012
Brazil 2010 2010 2010
Colombia 2010
European Union 2010 2010
Japan 2008 2008 2008
Korea 2010 2009
Mexico 2010 2010
Philippines 2009 2009 2011
South Africa 2010 2010 2010
Taiwan 2009
Turkey 2011

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked hybrid MON89034 X NK603 expresses two novel insecticidal proteins and a protein providing tolerance to the herbicide glyphosate. MON 89034 produces the Cry1A.105 and Cry2Ab2 proteins derived from Bacillus thuringiensis, which are active against lepidopteran insect pests. Tolerance to glyphosate is provided by the CP4 EPSPS protein, produced by the cp4 epsps gene from NK603. The novel traits of each parent line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for MON89034 and NK603.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1A.105 and Cry2Ab2 proteins are not enzymes and therefore do not affect plant metabolism. The CP4 EPSPS has high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. These proteins are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1A.105 chimeric cry1 delta-endotoxin IR CaMV 35S 5' untranslated leader from wheat chlorophyll a/b-binding protein Rice actin gene intron 3' untranslated region of wheat heat shock protein 17.3 1
cry2Ab cry2Ab delta-endotoxin IR FMV-35S - promoter from Figwort Mosaic Virus Hsp70 intron from maize heat shock protein gene. Transit peptide from maize RBC-small subunit. A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT enhanced CaMV 35S, maize HSP70 intron chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis subsp. kurstaki cry1Ab-Ac

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

MON89034 X NK603 (OECD identifier: MON-89Ø34-3 X MON-ØØ6Ø3-6) maize is an F1 hybrid resulting from the hybridization of maize inbred MON89034 (MON-89Ø34-3) with NK603 (MON-ØØ6Ø3-6). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Canada and Japan.

The stacked hybrid MON89034 X NK603 expresses two novel insecticidal proteins and a protein providing tolerance to the herbicide glyphosate. MON 89034 produces the Cry1A.105 and Cry2Ab2 proteins derived from Bacillus thuringiensis, which are active against lepidopteran insect pests. Tolerance to glyphosate is provided by the CP4 EPSPS protein, produced by the cp4 epsps gene from NK603. The novel traits of each parent line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for MON89034 and NK603.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1A.105 and Cry2Ab2 proteins are not enzymes and therefore do not affect plant metabolism. The CP4 EPSPS has high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. These proteins are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid.

Links to Further Information Expand


This record was last modified on Thursday, October 20, 2016