GM Crop Database

Database Product Description

MON810 x MON88017 (MON-ØØ81Ø-6 x MON-88Ø17-3)
Host Organism
Zea mays (Maize)
Trait

Herbicide tolerant, glyphosate; Insect resistant, Coleoptera; Insect resistant, Lepidoptera.

Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
Monsanto Company

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Canada 2006
Colombia 2011
European Union 2010 2010
Japan 2005 2006 2006
Korea 2006 2008
Mexico 2006 2006
Philippines 2006 2006
South Africa 2011 2011
Taiwan 2009
Turkey 2011

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked hybrid MON810 X MON88017 expresses three novel proteins: CP4 EPSPS which confers tolerance to the herbicide glyphosate, and the delta-endotoxins Cry1Ab, which confers resistance to the European Corn Borer and other lepidopterans, and Cry3Bb1 which confers resistance to Corn Rootworm. The insecticidal protein Cry1Ab, is produced by the cry1Ab gene from MON810. The insecticidal protein Cry3Bb1, is produced by the cry3Bb1 gene and the CP4 EPSPS protein is produced by the cp4 epsps gene both from MON88017. The novel traits of each parent line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for MON810 and MON88017.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1Ab and Cry3Bb1 proteins are not enzymes and therefore do not affect plant metabolism. The CP4 EPSPS has high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. These proteins are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Ab Cry1Ab delta-endotoxin (Btk HD-1) IR enhanced CaMV 35S, maize HSP70 intron None. Lost through 3' truncation during integration 1 Truncated
cry3Bb1 Cry3Bb1 delta-endotoxin IR CaMV 35S promoter with duplicated enhancer region 5' UTR from wheat chlorophyll a/b-binding protein; rice actin gene first intron 3' UTR from wheat heat shock protein (tahsp17 3') 1 functional synthetic
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT rice actin I promoter and intron sequences chloroplast transit peptide from A. thaliana A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 functional

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis subsp. kurstaki EC2.4.2.19

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Bacillus thuringiensis subsp. kumamotoensis cry3Bb1 While coleopterans are susceptible to oral doses of Cry3Bb1 protein, there is no evidence of toxic effects in laboratory mammals or birds. There are no significant mammalian toxins or allergens associated with the host organism.
Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Abstract Collapse

MON810 X MON88017 (OECD identifier: MON-ØØ81Ø-6 X MON-88Ø17-3) maize is an F1 hybrid resulting from the hybridization of maize inbred MON 810 (MON-ØØ81Ø-6) with MON88017 (MON-88Ø17-3). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Canada and Japan.

The stacked hybrid MON810 X MON88017 expresses three novel proteins: CP4 EPSPS which confers tolerance to the herbicide glyphosate, and the delta-endotoxins Cry1Ab, which confers resistance to the European Corn Borer and other lepidopterans, and Cry3Bb1 which confers resistance to Corn Rootworm. The insecticidal protein Cry1Ab, is produced by the cry1Ab gene from MON810. The insecticidal protein Cry3Bb1, is produced by the cry3Bb1 gene and the CP4 EPSPS protein is produced by the cp4 epsps gene both from MON88017. The novel traits of each parent line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for MON810 and MON88017.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1Ab and Cry3Bb1 proteins are not enzymes and therefore do not affect plant metabolism. The CP4 EPSPS has high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. These proteins are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid

Links to Further Information Expand


This record was last modified on Friday, August 11, 2017