GM Crop Database

Database Product Description

LLCotton25 x MON15985 (ACS-GHØØ1-3 x MON-15985-7)
Host Organism
Gossypium hirsutum (Cotton)
Trait

Herbicide tolerant, glufosinate ammonium; Insect resistant, Lepidoptera.

Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for fibre, livestock feed, and human consumption.

Product Developer
Bayer CropScience (Aventis CropScience(AgrEvo))

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Australia 2006 2006 2006
Japan 2006 2007 View
Korea 2007 2008
Mexico 2008 2008
New Zealand 2006
Taiwan 2015

Introduction Expand

This stacked cotton hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked cotton line LLCotton25 x MON15985 expresses three novel proteins: the enzyme phosphinothricin acetyl-transferase (PAT) and the delta-endotoxins Cry1Ac and Cry2Ab, both of which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm. The insecticidal proteins Cry1Ac and Cry2Ab are produced by the cry1Ac and cry2Ab genes respectively, both of which were originally isolated from Bacillus thuringiensis and are derived from MON15985. The bar gene, which codes for the production of PAT, is derived from LLCotton25. This enzyme acetylates glufosinate ammonium, rendering it inactive in the plant. The expression of the bar gene in LLCotton25 allows it to survive the otherwise lethal application of glufosinate ammonium. The bar gene was isolated from Streptomyces hygroscopius, a gram-positive soil bacterium. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new cotton line. For a full description of each parental line please refer to the individual product descriptions in the crop database for LLCotton25 and MON15985.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. Since neither Cry1Ac, nor Cry2Ab are enzymes, these proteins have no effect on plant metabolism. PAT has high affinity for its substrate L-Phosphinothricin, the active ingredient in glufosinate ammonium. PAT, Cry1Ac and Cry2Ab are therefore not expected to interact within, nor affect the metabolism of the stacked line.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
bar phosphinothricin N-acetyltransferase HT CaMV 35S A. tumefaciens nopaline synthase (nos) 3'-untranslated region
aad 3"(9)-O-aminoglycoside adenylyltransferase SM bacterial promoter Not expressed in plant tissues
cry1Ac Cry1Ac delta-endotoxin IR double enhanced CaMV 35S 3' poly(A) signal from soybean alpha subunit of the beta-conglycinin gene >=1 Truncated; Line 757: 1 complete T-DNA and 1 paritial T-DNA insertion event
nptII neomycin phosphotransferase II SM nopaline synthase (nos) from A. tumefaciens >=1 Native
cry2Ab Cry2Ab delta-endotoxin IR double enhanced CaMV 35S PetHSP70 leader sequence and the chloroplast transit peptide leader sequence (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 Modified to enhance in planta expression
uidA beta-D-glucuronidase SM double enhanced CaMV 35S A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 Native

Characteristics of Gossypium hirsutum (Cotton) Expand

Center of Origin Reproduction Toxins Allergenicity

Believed to originate in Meso-America (Peruvian-Ecuadorian-Bolivian region).

Generally self-pollinating, but can be cross-pollinating in the presence of suitable insect pollinators (bees). In the U.S., compatible species include G. hirsutum, G. barbadense, and G. tomentosum.

Gossypol in cottonseed meal.

Cotton is not considered to be allergenic, although there are rare, anecdotal reports of allergic reactions in the literature.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Streptomyces hygroscopicus bar S. hygroscopicus is ubiquitous in the soil and there have been no reports of adverse affects on humans, animals, or plants.
Bacillus thuringiensis subsp. kurstaki cry1Ac

Although target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals or bird given up to 10 µg protein / g body wt. There are no significant mammalian toxins or allergens associated with the host organism.

Bacillus thuringiensis subsp. kurstaki cry1Ab-Ac

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

LLCotton25 x MON15985 (OECD identifier: ACS- GHØØ1-3 x MON-15985-7) cotton is the product of the cross-breeding of the herbicide-tolerant cotton line LLCotton25 (OECD: ACS-GHØØ1-3) with the insect-resistant cotton line 15985 (MON-15985-7). This stacked cotton line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Japan and the Philippines.

The stacked cotton line LLCotton25 x MON15985 expresses three novel proteins: the enzyme phosphinothricin acetyl-transferase (PAT) and the delta-endotoxins Cry1Ac and Cry2Ab, both of which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm. The insecticidal proteins Cry1Ac and Cry2Ab are produced by the cry1Ac and cry2Ab genes respectively, both of which were originally isolated from Bacillus thuringiensis and are derived from MON15985. The bar gene, which codes for the production of PAT, is derived from LLCotton25. This enzyme acetylates glufosinate ammonium, rendering it inactive in the plant. The expression of the bar gene in LLCotton25 allows it to survive the otherwise lethal application of glufosinate ammonium. The bar gene was isolated from Streptomyces hygroscopius, a gram-positive soil bacterium. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new cotton line. For a full description of each parental line please refer to the individual product descriptions in the crop database for LLCotton25 and MON15985.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. Since neither Cry1Ac, nor Cry2Ab are enzymes, these proteins have no effect on plant metabolism. PAT has high affinity for its substrate L-Phosphinothricin, the active ingredient in glufosinate ammonium. PAT, Cry1Ac and Cry2Ab are therefore not expected to interact within, nor affect the metabolism of the stacked line.

Links to Further Information Expand


This record was last modified on Monday, August 7, 2017