GM Crop Database

Database Product Description

KK179 (MON-ØØ179-5)
Host Organism
Medicago sativa (Alfalfa)
Trait
Product Quality
Trait Introduction
Agrobacterium tumefaciens-mediated plant transformation.
Proposed Use

Production for livestock feed.

Product Developer
Monsanto Company and Forage Genetics International

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Australia 2014
Canada 2014 2014 2014
Japan 2015
Korea 2015 2015
Mexico 2015 2015
New Zealand 2014
United States 2013 2013 2014

Introduction Expand

In developing Reduced Lignin Alfalfa KK179, recombinant DNA techniques was used to introduce a fragment of the Caffeoyl CoA 3­Omethyltransferase (CCOMT) coding sequence. This fragment sequence was introduced in an inverted repeat, so that expression will produce a double stranded RNA that will, via RNA interference (RNAi), suppress endogenous CCOMT RNA expression levels. CCOMT is an essential part of the G lignin monomer biosynthesis pathway and suppression of this enzyme results in lower levels of the G lignin ((or Lucerne)) and overall lignin content in KK179.

Lignin is a non-carbohydrate phenolic polymer deposited in plant cell walls, particularly in the vascular tissue, and is a contributor to the quality of forage eaten by grazing animals. The genetic modification is supposed to provide growers with greater flexibility at harvest time, enabling the crop to be harvested at a later stage without appreciable loss of forage quality. KK179 is not intended to enter the food supply. However, a food approval was sought in some countries in case food exposure inadvertently occurs. 

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
CCOMT Caffeoyl CoA 3-O-methyltransferase PQ

P-Pal2 Promoter of the Pal2 gene from Phaseolus vulgaris encoding the phenylalanine ammonia-lyase

T-nos 3′ UTR sequence of the nopaline synthase (nos) gene from Agrobacterium tumefaciens pTi encoding NOS that directs polyadenylation

1

Inverted repeat

Characteristics of Medicago sativa (Alfalfa) Expand

Center of Origin Reproduction Toxins Allergenicity
Asia Minor, Transcaucasia, Turkmenistan, and Iran Outcrossing, insect-pollinated (bees). Hybridizes with members of the M. sativa complex; limited hybridization with M. glomerata. Seed dormancy variable, depending on amount of hard seed. Saponins, such as medicagenic acid, which cause bloat in ruminants; coumestrol and coumestan, phytoestrogens, which can cause reproductive problems in livestock. No known endogenous allergens

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Medicago sativa 5AT

Not applicable.

Modification Method Expand

Coming soon.

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Links to Further Information Expand


This record was last modified on Friday, February 24, 2017