GM Crop Database

Database Product Description

HCEM485

Host Organism

Zea mays (Maize)

Trait

Herbicide Tolerance

Trait Introduction

Direct DNA transfer system

Proposed Use

Production for human consumption and livestock feed.

Product Developer

Stine Biotechnology

Summary of Regulatory Approvals

Introduction Expand

Maize line HCEM485 was developed to be glyphosate herbicide-tolerant by introducing a 6.0 kb maize genomic fragment containing a double mutated form of the endogenous maize EPSPS encoding gene (2mEPSPS). The gene was introduced via aerosol beam injector, a naked DNA delivery tool. Except for the introduced mutations, the amino acid sequence of 2mEPSPS enzyme expressed in HCEM485 is identical to that of the EPSPS enzyme in the native wild-type maize. 

Summary of Introduced Genetic Elements Expand

Code	Name	Type	Promoter, other	Terminator	Copies	Form
epsps	5-enol-pyruvylshikimate-3-phosphate synthase	HT	5’ region of the maize (Z. mays)EPSPS-encoding gene containing native promoter sequences	3’ nontranslated region of the native maize (Z. mays) EPSPS-encoding gene	1	The mutations result in a glyphosate-tolerant form of the enzyme

Characteristics of Zea mays (Maize) Expand

Center of Origin	Reproduction	Toxins	Allergenicity
Mesoamerican region, now Mexico and Central America	Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.	No endogenous toxins or significant levels of antinutritional factors.	Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name	Gene	Pathogenicity
Zea mays	epsps	Not applicable.

Modification Method Expand

Coming soon.

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Links to Further Information Expand

Canada CFIA

• Decision Document DD2014-105 Determination of the Safety of Stine Seed Farm, Inc.'s Corn (Zea mays L.) Event HCEM485

United States, FDA

• Biotechnology Consultation Note to the File BNF No. 000106

United States, USDA

• Extended Determination of Nonregulated Status for HCEM485 Corn (Zea mays)
• Plant Pest Risk Assessment for HCEM485 Corn
• Request for Extension of Determination of Nonregulated Status to the Additional Regulated Article: Maize Line HCEM485
• Stine Seed Request (09-063-01p) for an Extension of a Determination of Nonregulated Status to HCEM485 - Final Environmental Assessment

This record was last modified on Thursday, December 15, 2016