GM Crop Database

Database Product Description

DAS-59122-7 x NK603 (DAS-59122-7 x MON-ØØ6Ø3-6)
Host Organism
Zea mays (Maize)
Trait
Resistance to coleopteran pests and glyphosate herbicide tolerance
Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
DOW AgroSciences LLC and Pioneer Hi-Bred International Inc.

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Canada 2005 2005 2005
European Union 2009 2009
Japan 2005 2006 2006
Korea 2006 2008
Mexico 2006 2006
Philippines 2006 2006
South Africa 2011 2011
Taiwan 2011 View
Turkey 2012

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked hybrid DAS-59122-7 X NK603 expresses four novel proteins: the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1, which are insecticidal to coleopterans such as the Corn Rootworm, together with phosphinothricin acetyl-transferase (PAT) and CP4 EPSPS which confer tolerance to the herbicides glufosinate ammonium and glyphosate, respectively. The CP4 EPSPS protein is produced by the cp4 epsps gene from NK603, and the insecticidal proteins Cry34Ab1 and Cry35Ab1, by the cry34Ab1 and cry35Ab1 genes from DAS-59122-7. The PAT protein is produced by the pat gene, also from DAS-59122-7. The novel traits of each parent line have therefore been combined, using traditional plant breeding methods, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for DAS-59122-7 and NK603.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry34Ab1 and Cry35Ab1 proteins are not enzymes and therefore do not affect plant metabolism. The CP4 EPSPS has high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. Similarly, the PAT protein has a very high affinity for L-Phosphinothricin, the active ingredient in the herbicide glufosinate ammonium. PAT, CP4 EPSPS, Cry34Ab1 and Cry35Ab1 are therefore not expected to interact within nor affect the metabolism of the stacked hybrid.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry34Ab1 Cry34Ab1 delta-endotoxin IR Zea mays ubiquitin gene promoter, intron and 5' UTR Solanum tuberosum proteinase inhibitor II (PINII) 1 functional Altered coding sequence for optimal expression in maize
cry35Ab1 Cry35Ab1 delta-endotoxin IR Triticum aestivum peroxidase gene root-preferred promoter Solanum tuberosum proteinase inhibitor II (PINII) 1 functional Altered coding sequence for optimal expression in maize
pat phosphinothricin N-acetyltransferase SM Cauliflower Mosaic Virus (CaMV) 35S Cauliflower Mosaic Virus (CaMV) 35S 1 functional
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT P-ract1/ract1 intron containing rice actin 1 promoter, transcription start site chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT enhanced CaMV 35S, maize HSP70 intron chloroplast transit peptide from A. thaliana EPSPS gene (CTP2) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 CP4 EPSPS gene modified for plant-preferred codons

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis strain PS149B1 cry34Ab1 While target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals, in birds or in non-target arthropods.
Bacillus thuringiensis strain PS149B1 cry34Ab1 While target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals, in birds or in non-target arthropods.
Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

DAS-59122-7 X NK603 (OECD identifier: DAS-59122-7 x MON-ØØ6Ø3-6) maize is an F1 hybrid resulting from the hybridization of the insect-resistant maize inbred line DAS-59122-7 and the herbicide-tolerant maize inbred line NK603 (MON-ØØ6Ø3-6). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Japan and the Philippines.

The stacked hybrid DAS-59122-7 X NK603 expresses four novel proteins: the insect control proteins (delta-endotoxins) Cry34Ab1 and Cry35Ab1, which are insecticidal to coleopterans such as the Corn Rootworm, together with phosphinothricin acetyl-transferase (PAT) and CP4 EPSPS which confer tolerance to the herbicides glufosinate ammonium and glyphosate, respectively. The CP4 EPSPS protein is produced by the cp4 epsps gene from NK603, and the insecticidal proteins Cry34Ab1 and Cry35Ab1, by the cry34Ab1 and cry35Ab1 genes from DAS-59122-7. The PAT protein is produced by the pat gene, also from DAS-59122-7. The novel traits of each parent line have therefore been combined, using traditional plant breeding methods, to produce this new hybrid. For a full description of each parent line please refer to the individual product descriptions in the crop database for DAS-59122-7 and NK603.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry34Ab1 and Cry35Ab1 proteins are not enzymes and therefore do not affect plant metabolism. The CP4 EPSPS has high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. Similarly, the PAT protein has a very high affinity for L-Phosphinothricin, the active ingredient in the herbicide glufosinate ammonium. PAT, CP4 EPSPS, Cry34Ab1 and Cry35Ab1 are therefore not expected to interact within nor affect the metabolism of the stacked hybrid.

Links to Further Information Expand

Canadian Food Inspection Agency European Food Safety Authority Health Canada Japanese Biosafety Clearing House, Ministry of Environment Mexican HEALTH SECRETARY Federal Commission for the Protection against Sanitary Risk Philippines Department of Agriculture, Bureau of Plant Industry Turkey's Ministry of Food, Agriculture and Livestock, General Directorate of Agricultural Research and Policies

This record was last modified on Thursday, October 29, 2015