GM Crop Database

Database Product Description

COT67B (SYN-IR67B-1)
Host Organism
Gossypium hirsutum (Cotton)

Insect resistant, Lepidoptera.

Trait Introduction
Agrobacterium tumefaciens-mediated plant transformation.
Proposed Use

Production for fibre, livestock feed, and human consumption.

Product Developer
Syngenta Seeds, Inc.

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Australia 2009
Canada 2011 2011
Japan 2012 2012 2012
Korea 2013
Mexico 2011 2011
New Zealand 2009
United States 2009 2009 2011

Introduction Expand

Insect-protected COT67B cotton has been genetically modified for protection against feeding damage caused by larvae of a number of insect pest species, including: Helicoverpa zea, cotton bollworm; and Heliothis virescens, tobacco budworm. Protection against these pests is achieved through expression in the plant of an insecticidal Cry protein, Cry1Ab, encoded by the full-length cry1Ab gene derived from Bacillus thuringiensis subspecies kurstaki HD-1.
Cry proteins exert their effect on the target insect by causing lysis of midgut epithelial cells, which leads to gut paralysis, cessation of feeding and eventual death of the larvae. The lysis of the midgut epithelial cells is mediated by the binding of the activated Cry protein to specialized receptors on these cells.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
aph4 hygromycin-B phosphotransferase SM promoter and first intron of A. thaliana ubiquitin-3 gene A. tumefaciens nopaline synthase (nos) 3'-untranslated region None. Lost through breeding process.
flcry1Ab Cry1Ab delta-endotoxin IR Promoter region from the actin-2 gene of Arabidopsis thaliana and its intron. A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1

Characteristics of Gossypium hirsutum (Cotton) Expand

Center of Origin Reproduction Toxins Allergenicity

Believed to originate in Meso-America (Peruvian-Ecuadorian-Bolivian region).

Generally self-pollinating, but can be cross-pollinating in the presence of suitable insect pollinators (bees). In the U.S., compatible species include G. hirsutum, G. barbadense, and G. tomentosum.

Gossypol in cottonseed meal.

Cotton is not considered to be allergenic, although there are rare, anecdotal reports of allergic reactions in the literature.

Modification Method Expand

Cotton event COT67B was produced via Agrobacterium-mediated transformation of the Coker 312 cultivar using two plasmid vectors, pNOV4641 and pNOV1914. The T-DNA region of pNOV4641 contained a single copy of the flcry1Ab gene derived from B. thuringiensis subsp. kurstaki strain HD-1. The T-DNA region of pNOV1914 contained a single copy of the selectable marker gene aph4, which encodes hygromycin-B phosphotransferase. Expression of hygromycin-B phosphotransferase confers resistance to the antibiotic hygromycin, which was used for selection of transformed tissue during the initial tissue culture phase of transformation. However, once transformants were identified, traditional breeding was used to identify segregants containing flcry1Ab but lacking the aph4 gene, including elements from its plasmid backbone. The aph4 gene and related regulatory sequences are no longer present in cotton event COT67B.

Links to Further Information Expand

Canadian Food Inspection Agency Food Standards Australia New Zealand Health Canada's Food Directorate Japan Ministry of Health, Labor and Welfare Mexico Health Secretary Federal Commission for the Protection against Sanitary Risk Summary Assessment Safety United States (USDA) United States Environmental Protection Agency United States Food and Drug Administration

This record was last modified on Wednesday, March 2, 2016