GM Crop Database

Database Product Description

BT11xMIR162xTC1507xGA21 (SYN-BTØ11-1xSYN-IR162-4xDAS-Ø15Ø7-1xMON-ØØØ21-9)
Host Organism
Zea mays (Maize)
Trait

Multiple herbicide resistance, Insect resistance

Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
Syngenta Seeds, Inc.

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Argentina 2014 2014 2014
Colombia 2016
Japan 2010 2010 2011
Korea 2012 2012
Mexico 2011 2011
Philippines 2010 2010
South Africa 2011 2011
Taiwan 2011

Introduction Expand

Coming soon.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Ab Cry1Ab delta-endotoxin IR

35S promoter obtained as DdeI-DdeI fragment derived from cauliflower mosaic virus (CaMV) CM1841 strain

NOS - 3' untranslated region of nopaline synthase (NOS) gene of Agrobacterium tumefaciens, which contains a transcription terminator and a signal for polyadenylation of mRNA

Partial deletion of the C-terminal code region which is independent from the insecticidal activity of the Cry1Ab protein and modifying some nucleotide sequences to change the contents of GC and enhance its expression level in plants

cry3A cry3A delta-endotoxin IR

MTL promoter derived from the metallothionein gene of maize. The MTL promoter is used to define the start of transcription of target genes in the roots

NOS the terminator region of the nopaline synthase gene of Agrobacterium tumefaciens, which terminates transcription and induces polyadenylation

Modifying some nucleotide sequences to change the contents of GC and enhance its expression level in plants and transferring cathepsin G protease recognition sequence to enhance the activity against Corn Rootworm

epsps epsps 5-enol-pyruvylshikimate-3-phosphate synthase HT

Act promoter derived from the rice actin 1 gene, including up to the first intron region

NOS polyadenylation sequence of the nopaline synthase (NOS) gene from Agrobacterium tumefaciens, terminating transcription

The 102nd amino acid threonine in the wild-type EPSPS amino acid sequence was modified to isoleucine, and the 106th proline modified to serine

vip3A(a) Vip3A(a) vegetative insecticidal protein IR

ZmUbiInt promoter region from Z. mays polyubiquitin gene which contains the first intron (1,010bp)

35S polyadenylation sequence derived from the cauliflower mosaic virus 35S RNA

The amino acid at position 284 in the amino acid sequence was substituted to glutamine from lysine. In addition, in the modified Vip3A protein expressed in MIR162, 129th methionine was substituted by isoleucine by mutation of forming transformant, as well as 284th amino acid substitution

pmi pmi mannose-6-phosphate isomerase SM

ZmUbiInt promoter region from Z. mays polyubiquitin gene which contains the first intron (1,010bp)

NOS the terminator region of the nopaline synthase gene of Agrobacterium tumefaciens, which terminates transcription and induces polyadenylation

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis subsp. kurstaki cry1Ab-Ac

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Bacillus thuringiensis subsp. Tenebrionis cry3A While beetles and other coleopterans are susceptible to oral doses of Cry3A protein, there is no evidence of toxic effects in laboratory mammals or birds. There are no significant mammalian toxins or allergens associated with the host organism.
Zea mays epsps

Not applicable.

Bacillus thuringiensis strain AB88 5AT

While target lepidopteran insects are susceptible to oral doses of VIP proteins, there is no evidence of toxic effects in laboratory mammals, in birds, and in non-target arthropods, including beneficial insects.

Escherichia coli (K-12) hyg

Escherichia coli is one of a number of microorganisms which are normal inhabitants of the colons of virtually all warm-blooded mammals. Ecoli belongs to the taxonomic family known asEnterobacteriaceae, which is one of the best-defined groups of bacteria. The strain Ecoli K-12 is a debilitated strain which does not normally colonize the human intestine. It has also been shown to survive poorly in the environment, has a history of safe commercial use, and is not known to have adverse effects on microorganisms or plants. 

Modification Method Expand

Coming soon.

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Links to Further Information Expand


This record was last modified on Tuesday, August 16, 2016