GM Crop Database

Database Product Description

BT11xMIR162xTC1507xGA21 (SYN-BTØ11-1xSYN-IR162-4xDAS-Ø15Ø7-1xMON-ØØØ21-9)

Host Organism

Zea mays (Maize)

Trait

Multiple herbicide resistance, Insect resistance

Trait Introduction

Traditional plant breeding and selection

Proposed Use

Production for human consumption and livestock feed.

Product Developer

Syngenta Seeds, Inc.

Summary of Regulatory Approvals

Introduction Expand

Coming soon.

Summary of Introduced Genetic Elements Expand

Code	Name	Type	Promoter, other	Terminator	Copies	Form
cry1Ab	Cry1Ab delta-endotoxin	IR	35S promoter obtained as DdeI-DdeI fragment derived from cauliflower mosaic virus (CaMV) CM1841 strain	NOS - 3' untranslated region of nopaline synthase (NOS) gene of Agrobacterium tumefaciens, which contains a transcription terminator and a signal for polyadenylation of mRNA		Partial deletion of the C-terminal code region which is independent from the insecticidal activity of the Cry1Ab protein and modifying some nucleotide sequences to change the contents of GC and enhance its expression level in plants
cry3A	cry3A delta-endotoxin	IR	MTL promoter derived from the metallothionein gene of maize. The MTL promoter is used to define the start of transcription of target genes in the roots	NOS the terminator region of the nopaline synthase gene of Agrobacterium tumefaciens, which terminates transcription and induces polyadenylation		Modifying some nucleotide sequences to change the contents of GC and enhance its expression level in plants and transferring cathepsin G protease recognition sequence to enhance the activity against Corn Rootworm
epsps	epsps 5-enol-pyruvylshikimate-3-phosphate synthase	HT	Act promoter derived from the rice actin 1 gene, including up to the first intron region	NOS polyadenylation sequence of the nopaline synthase (NOS) gene from Agrobacterium tumefaciens, terminating transcription		The 102nd amino acid threonine in the wild-type EPSPS amino acid sequence was modified to isoleucine, and the 106th proline modified to serine
vip3A(a)	Vip3A(a) vegetative insecticidal protein	IR	ZmUbiInt promoter region from Z. mays polyubiquitin gene which contains the first intron (1,010bp)	35S polyadenylation sequence derived from the cauliflower mosaic virus 35S RNA		The amino acid at position 284 in the amino acid sequence was substituted to glutamine from lysine. In addition, in the modified Vip3A protein expressed in MIR162, 129th methionine was substituted by isoleucine by mutation of forming transformant, as well as 284th amino acid substitution
pmi	pmi mannose-6-phosphate isomerase	SM	ZmUbiInt promoter region from Z. mays polyubiquitin gene which contains the first intron (1,010bp)	NOS the terminator region of the nopaline synthase gene of Agrobacterium tumefaciens, which terminates transcription and induces polyadenylation

Characteristics of Zea mays (Maize) Expand

Center of Origin	Reproduction	Toxins	Allergenicity
Mesoamerican region, now Mexico and Central America	Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.	No endogenous toxins or significant levels of antinutritional factors.	Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name	Gene	Pathogenicity
Bacillus thuringiensis subsp. kurstaki	cry1Ab-Ac	While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.
Bacillus thuringiensis subsp. Tenebrionis	cry3A	While beetles and other coleopterans are susceptible to oral doses of Cry3A protein, there is no evidence of toxic effects in laboratory mammals or birds. There are no significant mammalian toxins or allergens associated with the host organism.
Zea mays	epsps	Not applicable.
Bacillus thuringiensis strain AB88	5AT	While target lepidopteran insects are susceptible to oral doses of VIP proteins, there is no evidence of toxic effects in laboratory mammals, in birds, and in non-target arthropods, including beneficial insects.
Escherichia coli (K-12)	hyg	Escherichia coli is one of a number of microorganisms which are normal inhabitants of the colons of virtually all warm-blooded mammals. E. coli belongs to the taxonomic family known asEnterobacteriaceae, which is one of the best-defined groups of bacteria. The strain E. coli K-12 is a debilitated strain which does not normally colonize the human intestine. It has also been shown to survive poorly in the environment, has a history of safe commercial use, and is not known to have adverse effects on microorganisms or plants.

Modification Method Expand

Coming soon.

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Links to Further Information Expand

Argentina Ministery of Agriculture , Livestock and Fisheries

• Visto el Expediente No S05:0562166/2013 del Registro del Ministeril de Agricultura, Ganaderia y Pesca
  [PDF Size: 169.15K bytes]

Japan Ministry of Health, Labor and Welfare

• Biological Diversity Risk Assessment Report for Maize resistant to Lepidoptera and tolerant to glufosinate herbicide and glyphosate herbicide (modified cry1Ab, modified vip3A, cry1F, pat, mEPSPS, Zea mays subsp. mays (L.) Ilt is) (Bt11×MIR162×B.t. Cry1F maize line 1507×GA21)
  [PDF Size: 331.48K bytes]

Philippines

• Determination of the Safety of Syngenta’s Stacked trait product corn: Bt11 x MIR162 x TC1507 x GA21 for Direct use as Food, Feed, or Processing
  [PDF Size: 507.36K bytes]

This record was last modified on Tuesday, August 16, 2016