GM Crop Database

Database Product Description

Bt11 x MIR604 (SYN-BTØ11-1xSYN-IR6Ø4-5)
Host Organism
Zea mays (Maize)
Trait

Herbicide tolerant, glufosinate ammonium; Insect resistant, Coleoptera; Insect resistant, Lepidoptera.

Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
Syngenta Seeds, Inc.

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Canada 2007
Colombia 2012
European Union 2011 2011
Japan 2007 2008 2008
Korea 2007 2007
Mexico 2007 2007
Philippines 2007 2007
South Africa 2011 2011
Taiwan 2009
Turkey 2015

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked hybrid BT11 x MIR604 expresses four novel proteins: the delta-endotoxin Cry1Ab which confers resistance to the European Corn Borer and other lepidopterans, the PAT protein which confers tolerance to the herbicide glufosinate ammonium, the delta-endotoxin mCry3A which confers resistance to corn rootworm (Diabrotica species), and the PMI protein which allows growth on mannose as a carbon source and is used as a selectable marker. The insecticidal protein Cry1Ab is produced by the cry1Ab gene and PAT is produced by the pat gene, both from BT11, the insecticidal protein mCry3A is produced by the mcry3A gene and PMI is produced by the pmi gene, both from MIR604. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parental line please refer to the individual product descriptions in the crop database for BT11 and MIR604.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1Ab and mCry3A proteins are not enzymes and therefore do not affect plant metabolism. PAT has a high affinity for L-Phosphinothricin, the active ingredient in glufosinate ammonium and PMI has a high affinity for mannose-6-phosphate, converting it to fructose-6-phosphate which is directly utilised by the plant. PMI, PAT, Cry1Ab and mCry3A are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Ab Cry1Ab delta-endotoxin (Btk HD-1) IR CaMV 35S IVS 6 intron from the maize alcohol dehydrogenase gene A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 Truncated, modified
pat phosphinothricin N-acetyltransferase HT CaMV 35S IVS 2 intron from the maize alcohol dehydrogenase gene A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 Modified for enhanced expression
pmi mannose-6-phosphate isomerase SM ZmUbiInt (Zea mays poly-ubiquitin gene promoter and first intron) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1
mcry3A Cry3A delta-endotoxin IR promoter derived from the metallothionein-like gene from Zea mays A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 Modified to enhance expression in maize

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis subsp. kurstaki EC2.4.2.19

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

BT11 x MIR604 (OECD identifier: SYN-BTØ11-1 x SYN-IR6Ø4-5) maize is an F1 hybrid resulting from the hybridization of the lepidopteran-resistant and glufosinate-tolerant maize line BT11 (SYN-BTØ11-1) and the coleopteran-resistant maize line MIR604 (SYN-IR6Ø4-5). This stacked maize hybrid is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Japan and Korea.

The stacked hybrid BT11 x MIR604 expresses four novel proteins: the delta-endotoxin Cry1Ab which confers resistance to the European Corn Borer and other lepidopterans, the PAT protein which confers tolerance to the herbicide glufosinate ammonium, the delta-endotoxin mCry3A which confers resistance to corn rootworm (Diabrotica species), and the PMI protein which allows growth on mannose as a carbon source and is used as a selectable marker. The insecticidal protein Cry1Ab is produced by the cry1Ab gene and PAT is produced by the pat gene, both from BT11, the insecticidal protein mCry3A is produced by the mcry3A gene and PMI is produced by the pmi gene, both from MIR604. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid. For a full description of each parental line please refer to the individual product descriptions in the crop database for BT11 and MIR604.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1Ab and mCry3A proteins are not enzymes and therefore do not affect plant metabolism. PAT has a high affinity for L-Phosphinothricin, the active ingredient in glufosinate ammonium and PMI has a high affinity for mannose-6-phosphate, converting it to fructose-6-phosphate which is directly utilised by the plant. PMI, PAT, Cry1Ab and mCry3A are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid.

Links to Further Information Expand


This record was last modified on Friday, August 4, 2017