GM Crop Database

Database Product Description

Bt11 x MIR162 (SYN-BTØ11-1xSYN-IR162-4)
Host Organism
Zea mays (Maize)
Resistance to a range of lepidopteran insect pests; phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium.
Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for human consumption and livestock feed.

Product Developer
Syngenta Seeds, Inc.

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Argentina 2014 2014 2014
Colombia 2017
European Union 2016 2016
Japan 2010 2010 2010
Korea 2016 2015
Philippines 2013 2013
South Africa 2015 2015
Taiwan 2015
United States 2009 View

Introduction Expand

This stacked maize hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked hybrid BT11 x MIR162 expresses four novel proteins: the delta-endotoxin Cry1Ab which confers resistance to the European Corn Borer and other lepidopterans, the PAT protein which confers tolerance to the herbicide glufosinate ammonium, the vegitative insecticidal protein (VIP) 3Aa from Bacillus thuringiensis that is highly toxic to H. zea, S. frugiperda, A. ipsilon, and S. albicosta larvae, and the PMI protein which allows growth on mannose as a carbon source and is used as a selectable marker. The insecticidal protein Cry1Ab is produced by the cry1Ab gene and PAT is produced by the pat gene, both from BT11; the VIP3Aa protein is produced by the vip3Aa20 gene and PMI is produced by the pmi gene, both from MIR162. The novel traits of each parental line have been combined, through traditional plant breeding, to produce this new hybrid.

While crop losses attributable to O. nubilalis and Diabrotica infestations have been well characterized and are significant, there is not as much quantitative information available on the economic impacts of other major insect pests of maize, specifically H. zea, S. frugiperda, A. ipsilon, and S. albicosta. These pests are not as widespread as corn borers and rootworms; however, crop infestations by these leaf and ear-feeding pests can be very costly to growers, as they have the potential to signifi cantly lower grain yield and quality. Conventional insecticide applications are an option for reducing feeding damage caused by these insects; however, most growers do not treat their crops to control these pests because of cost and limited effectiveness of the chemical agents. Currently available novel varieties are not as efficacious against these lepidopteran insects as they are against O. nubilalis. For example, Bt11 maize containing the Cry1Ab toxin provides only limited or no protection against feeding damage caused by H. zea, S. frugiperda, A. ipsilon, and S. albicosta. The combination of the Cry1Ab protein from BT11 and the Vip3Aa protein from MIR162 can provide growers the means of protecting their maize crops from damage caused by a broad range of lepidopteran pests.

For a full description of each parental line please refer to the individual product descriptions in the crop database for BT11 and MIR162.

The inserted genes and their gene products have a history of safe use, and have undergone review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked hybrid. The Cry1Ab and VIP3Aa proteins do not affect plant metabolism. PAT has a high affinity for L-Phosphinothricin, the active ingredient in glufosinate ammonium and PMI has a high affinity for mannose-6-phosphate, converting it to fructose-6-phosphate which is directly utilised by the plant. PMI, PAT, Cry1Ab and Vip3Aa are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Ab Cry1Ab delta-endotoxin (Btk HD-1) IR CaMV 35S IVS 6 intron from the maize alcohol dehydro-genase gene A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 Truncated, modified
pat phosphinothricin N-acetyltransferase HT CaMV 35S IVS 2 intron from the maize alcohol dehydro-genase gene A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1 Modified for enhanced expression
vip3Aa20 vegetative insecticidal protein IR ZmUbiInt (Zea mays polyubiquitin gene promoter and first intron). CaMV 35S 3' polyadenylation signal 1 native
pmi mannose-6-phosphate isomerase SM ZmUbiInt (Zea mays poly-ubiquitin gene promoter and first intron) A. tumefaciens nopaline synthase (nos) 3'-untranslated region 1

Characteristics of Zea mays (Maize) Expand

Center of Origin Reproduction Toxins Allergenicity

Mesoamerican region, now Mexico and Central America

Cross-pollination via wind-borne pollen is limited, pollen viability is about 30 minutes. Hybridization reported with teosinte species and rarely with members of the genus Tripsacum.

No endogenous toxins or significant levels of antinutritional factors.

Although some reported cases of maize allergy, protein(s) responsible have not been identified.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis subsp. kurstaki EC2.4.2.19

While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds given up to 10 µg protein/g body weight.

Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Bacillus thuringiensis strain AB88 5AT

While target lepidopteran insects are susceptible to oral doses of VIP proteins, there is no evidence of toxic effects in laboratory mammals, in birds, and in non-target arthropods, including beneficial insects.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Links to Further Information Expand

This record was last modified on Friday, August 4, 2017