GM Crop Database

Database Product Description

281-24-236 x 3006-210-23 x MON88913 (DAS-24236-5 x DAS-21Ø23-5 x MON-88913-8)
Host Organism
Gossypium hirsutum (Cotton)
Trait
Resistance to lepidopteran pests and glyphosate herbicide tolerance
Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for fibre, livestock feed, and human consumption.

Product Developer
DOW AgroSciences LLC and Pioneer Hi-Bred International Inc.

Summary of Regulatory Approvals

Country Food Feed Environment Notes
European Union 2017 2017
Japan 2006 2006
Korea 2006
Korea 2008
Mexico 2006 2006

Introduction Expand

This stacked cotton hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

The stacked cotton line WideStrike™ X MON88913 expresses four novel proteins: the delta-endotoxins Cry1F and Cry1Ac, which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm, the CP4 EPSPS protein, which confers tolerance to the herbicide glyphosate, and the PAT protein, which confers tolerance to glufosinate ammonium. The insecticidal proteins Cry1F and Cry1Ac are produced by the cry1F gene and cry1Ac genes respectively, both of which are from WideStrike™. The cotton line WideStrike™ was produced by crossing line 281-24-236 (OECD identifier: DAS-24236-5) with line 3006-210-23 (OECD identifier: DAS-21023-5). The PAT protein in WideStrike™ is produced by the pat gene: this expressed novel protein was intended solely for use as a selectable marker during plant transformation. The CP4 EPSPS protein is from MON88913 which contains two copies of the cp4 epsps gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage. For a full description of each parental line please refer to the individual product descriptions in the crop database for 281-24-236 and 3006-210-23 (the parental lines of WideStrike™ cotton) and MON88913.

The inserted genes and their gene products have a history of safe use, and have undergone prior review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in this stacked line. Since neither Cry1F, nor Cry1Ac are enzymes, these proteins have no effect on plant metabolism. The CP4 EPSPS has a very high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. The PAT protein has a very high affinity for L-Phosphinothricin, the active ingredient in the herbicide glufosinate ammonium. CP4 EPSPS, Cry1F, Cry1Ac, and PAT are therefore not expected to interact within, nor affect the metabolism of the stacked line.

The EPA conducted an environmental hazard assessment of WideStrike™ cotton. Data on the effects of the Cry1F and Cry1Ac were assessed separately, and in combination to detect possible synergistic effects. No synergistic effects were observed, nor any increase in the host range of non-target organisms, from the stacking of both Cry proteins. No harmful effects to aquatic and terrestrial wildlife were observed, and if was concluded that the cultivation of the stacked line would not be hazardous to non-target terrestrial, aquatic and soil organisms.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Ac Cry1Ac delta-endotoxin IR

ubiquitin 1 (Zea mays)

3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens)

1 functional;

Cry1Ac active insecticidal core and non-toxic portions of the Cry1Ab1 and Cry1Ca3 proteins.  Sequence modified for optimal in planta expression.

pat phosphinothricin N-acetyltransferase SM mannopine synthase (d mas 2') promoter from pTi15955 four copies of the octopine synthase (4OCS) enhancer from pTiAch5 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; Altered coding sequence for optimal expression in plant cells.
cry1F cry1F delta-endotoxin IR mannopine synthase (d mas 2') promoter from pTi15955 four copies of the octopine synthase (4OCS) enhancer from pTiAch5 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; Cry1F active insecticidal core and non-toxic portions of the Cry1Ab1 and Cry1Ca3 proteins. Sequence modified for optimal in planta expression.
pat phosphinothricin N-acetyltransferase SM ubiquitin (ubi) ZM (Zea mays) promoter and the first exon and intron 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; 1 partial, non-expressed; Altered coding sequence for optimal expression in plant cells.
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT P-FMV/TSF1: chimeric promoter containing A. thaliana tsf1 promoter elongation factor EF-1 alpha; Figwort Mosaic Virus 35S promoter enhancer; A. thaliana tsf1 leader and intron; chloroplast transit peptide from A. thaliana Pisum sativum T-E9 DNA containing 3’ UTR of Rubisco small subunit E9 gene 1 functional
CP4 epsps 5-enolpyruvyl shikimate-3-phosphate synthase HT P-35S/ACT8: chimeric promoter from A. thaliana act8 f Cauliflower Mosaic Virus 35S promoter enhancer; A. thaliana act8 leader and intron; chloroplast transit peptide from A. thaliana Pisum sativum T-E9 DNA containing 3’ UTR of Rubisco small subunit E9 gene 1 functional

Characteristics of Gossypium hirsutum (Cotton) Expand

Center of Origin Reproduction Toxins Allergenicity

Believed to originate in Meso-America (Peruvian-Ecuadorian-Bolivian region).

Generally self-pollinating, but can be cross-pollinating in the presence of suitable insect pollinators (bees). In the U.S., compatible species include G. hirsutum, G. barbadense, and G. tomentosum.

Gossypol in cottonseed meal.

Cotton is not considered to be allergenic, although there are rare, anecdotal reports of allergic reactions in the literature.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis subsp. kurstaki cry1Ac

Although target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals or bird given up to 10 µg protein / g body wt. There are no significant mammalian toxins or allergens associated with the host organism.

Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Bacillus thuringiensis var. aizawai cry1F While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds.
Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Agrobacterium tumefaciens strain CP4 CP4 epsps

Agrobacterium tumefaciens is a common soil bacterium that is responsible for causing crown gall disease in susceptible plants. There have been no reports of adverse effects on humans or animals.

Modification Method Expand

Coming soon. 

Characteristics of the Modification Expand

Coming soon. 

Environmental Safety Considerations Expand

Coming soon. 

Food and/or Feed Safety Considerations Expand

Coming soon. 

Abstract Collapse

WideStrike™ x MON88913 (OECD identifier: DAS-21023-5 x DAS-24236-5 x MON-88913-8) was produced by crossing WideStrike™ insect resistant cotton with the herbicide-tolerant cotton line MON88913. This stacked cotton line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Canada and Japan. The United States Environmental Protection Agency (EPA) reviews stacked events when these are the result of stacking two or more plant-incorporated insecticidal proteins, such as WideStrikeTM cotton.

The stacked cotton line WideStrike™ X MON88913 expresses four novel proteins: the delta-endotoxins Cry1F and Cry1Ac, which confer resistance to the lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm, the CP4 EPSPS protein, which confers tolerance to the herbicide glyphosate, and the PAT protein, which confers tolerance to glufosinate ammonium. The insecticidal proteins Cry1F and Cry1Ac are produced by the cry1F gene and cry1Ac genes respectively, both of which are from WideStrike™. The cotton line WideStrike™ was produced by crossing line 281-24-236 (OECD identifier: DAS-24236-5) with line 3006-210-23 (OECD identifier: DAS-21023-5). The PAT protein in WideStrike™ is produced by the pat gene: this expressed novel protein was intended solely for use as a selectable marker during plant transformation. The CP4 EPSPS protein is from MON88913 which contains two copies of the cp4 epsps gene to confer tolerance to glyphosate later in the growing season, specifically after the fifth true leaf stage. For a full description of each parental line please refer to the individual product descriptions in the crop database for 281-24-236 and 3006-210-23 (the parental lines of WideStrike™ cotton) and MON88913.

The inserted genes and their gene products have a history of safe use, and have undergone prior review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in this stacked line. Since neither Cry1F, nor Cry1Ac are enzymes, these proteins have no effect on plant metabolism. The CP4 EPSPS has a very high affinity for its substrates phosphoenolpyruvate (PEP) and shikimate-3-phosphate, which are part of the shikimate metabolic pathway. The PAT protein has a very high affinity for L-Phosphinothricin, the active ingredient in the herbicide glufosinate ammonium. CP4 EPSPS, Cry1F, Cry1Ac, and PAT are therefore not expected to interact within, nor affect the metabolism of the stacked line.

The EPA conducted an environmental hazard assessment of WideStrike™ cotton. Data on the effects of the Cry1F and Cry1Ac were assessed separately, and in combination to detect possible synergistic effects. No synergistic effects were observed, nor any increase in the host range of non-target organisms, from the stacking of both Cry proteins. No harmful effects to aquatic and terrestrial wildlife were observed, and if was concluded that the cultivation of the stacked line would not be hazardous to non-target terrestrial, aquatic and soil organisms.

Links to Further Information Expand

Japanese Biosafety Clearing House, Ministry of Environment

This record was last modified on Friday, August 4, 2017