GM Crop Database

Database Product Description

281-24-236 x 3006-210-23 (DAS-24236-5 x DAS-21Ø23-5)
Host Organism
Gossypium hirsutum (Cotton)
Trade Name
WideStrike™
Trait

Resistance to lepidopteran pests and glufosinate ammonium herbicide tolerance.

Trait Introduction
Traditional plant breeding and selection
Proposed Use

Production for fibre, livestock feed, and human consumption.

Product Developer
DOW AgroSciences LLC

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Australia 2005 2009
Brazil 2009 2009 2009
European Union 2011 2011
Japan 2005 2005
Korea 2005 2008
Mexico 2004 2004
New Zealand 2005
Taiwan 2015
United States 2004 2004 2004

Introduction Expand

This stacked cotton hybrid is a product of traditional plant breeding, and is therefore not automatically subject to regulation in all countries, unlike transgenic plants resulting from recombinant-DNA technologies. The approvals table above does not include entries from these countries. Other countries may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment, and these countries’ decisions are reflected in the approvals table.

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
cry1Ac Cry1Ac delta-endotoxin IR ubiquitin 1 (Zea mays) 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; Cry1Ac active insecticidal core and non-toxic portions of the Cry1Ab1 and Cry1Ca3 proteins. Sequence modified for optimal in planta expression.
pat phosphinothricin N-acetyltransferase SM mannopine synthase (d mas 2') promoter from pTi15955 four copies of the octopine synthase (4OCS) enhancer from pTiAch5 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; Altered coding sequence for optimal expression in plant cells.
cry1F cry1F delta-endotoxin IR mannopine synthase (d mas 2') promoter from pTi15955 four copies of the octopine synthase (4OCS) enhancer from pTiAch5 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; Cry1F active insecticidal core and non-toxic portions of the Cry1Ab1 and Cry1Ca3 proteins. Sequence modified for optimal in planta expression.
pat phosphinothricin N-acetyltransferase SM ubiquitin (ubi) ZM (Zea mays) promoter and the first exon and intron 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) 1 functional; 1 partial, non-expressed; Altered coding sequence for optimal expression in plant cells.

Characteristics of Gossypium hirsutum (Cotton) Expand

Center of Origin Reproduction Toxins Allergenicity

Believed to originate in Meso-America (Peruvian-Ecuadorian-Bolivian region).

Generally self-pollinating, but can be cross-pollinating in the presence of suitable insect pollinators (bees). In the U.S., compatible species include G. hirsutum, G. barbadense, and G. tomentosum.

Gossypol in cottonseed meal.

Cotton is not considered to be allergenic, although there are rare, anecdotal reports of allergic reactions in the literature.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Bacillus thuringiensis subsp. kurstaki cry1Ac

Although target insects are susceptible to oral doses of Bt proteins, there is no evidence of toxic effects in laboratory mammals or bird given up to 10 µg protein / g body wt. There are no significant mammalian toxins or allergens associated with the host organism.

Streptomyces viridochromogenes pat

S. viridochromogenes is ubiquitous in the soil. It exhibits very slight antimicrobial activity, is inhibited by streptomycin, and there have been no reports of adverse affects on humans, animals, or plants.

Bacillus thuringiensis var. aizawai cry1F While target insects are susceptible to oral doses of Bt proteins, no evidence of toxic effects in laboratory mammals or birds.

Abstract Collapse

WideStrike™ cotton (OECD identifier: DAS-21Ø23-5 x DAS-24236-5) was produced by cross-breeding two insect-resistant cotton lines: 281-24-236 (OECD identifier: DAS-24236-5) and 3006-210-23 (OECD identifier: DAS-21Ø23-5). Each of these lines expresses an insecticidal protein. This stacked cotton line is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment. Examples of jurisdictions that require either notification or information about stacked hybrids prior to their release into the environment, and for use in human food and livestock feed are Canada and Japan. The United States Environmental Protection Agency (EPA) reviews stacked events when these are the result of stacking two or more plant-incorporated insecticidal proteins, such as WideStrike™ cotton. WideStrike™ expresses two novel proteins: Cry1F and Cry1Ac, delta-endotoxins which confers resistance to lepidopteran pests of cotton, such as the cotton bollworm, pink bollworm and tobacco budworm. The insecticidal protein Cry1F is produced by the cry1F gene from cotton line 281-24-236, and Cry1Ac is produced by the cry1Ac gene from the cotton line 3006-210-23. The pat gene is also expressed in WideStrike™. This gene produces the PAT protein (phosphinothricin acetyltransferate) which confers resistance to the herbicide glufosinate ammonium, and inserted solely to be used as a selectable marker during the transformation that led to the production of 281-24-23 and 3006-210-23. For a full description of each parental line, please refer to the individual product descriptions in the crop database for 281-24-236 and 3006-210-23. The inserted genes and their gene products in WideStrike™ cotton have a history of safe use, and have undergone prior review and approval by several regulatory agencies. No interactions among the gene products or negative synergistic effects are expected in the stacked line. Since neither Cry1F, nor Cry1Ac, have enzymatic activity, these proteins have no effect on plant metabolism. The PAT protein has a very high affinity for L-Phosphinothricin, the active ingredient in the herbicide glufosinate ammonium. Cry1F, Cry1Ac and PAT are therefore not expected to interact within, nor affect the metabolism of the stacked hybrid. The EPA conducted an environmental hazard assessment of WideStrike™ cotton. Data on the effects of the Cry1F and Cry1Ac were assessed separately, and in combination to detect possible synergistic effects. No synergistic effects were observed, nor any increase in the host range of non-target organisms, from the stacking of both Cry proteins. No harmful effects to aquatic and terrestrial wildlife were observed, and if was concluded that the cultivation of the stacked line would not be hazardous to non-target terrestrial, aquatic and soil organisms.

Links to Further Information Expand

Australian Government, Department of Health and Ageing Comissão Técnica Nacional de Biossegurança - CTNBio (Brazil) European Food Safety Authority (EFSA) Food Standards Australia New Zealand Japanese Biosafety Clearing House, Ministry of Environment U.K. Advisory Committee on Releases to the Environment U.K. Department for Environment, Food and Rural Affairs U.S. Environmental Protection Agency United States Department of Agriculture United States Food and Drug Administration

This record was last modified on Monday, August 8, 2016