GM Crop Database

Database Product Description

25958 (IFD-25958-3)
Host Organism
Dianthus caryophyllus (Carnation)
Pigment Modification
Trait Introduction
Agrobacterium tumefaciens-mediated plant transformation.
Proposed Use

Production for the cut-flower industry.

Product Developer
Suntory Limited

Summary of Regulatory Approvals

Country Food Feed Environment Notes
Australia 2015 View
Colombia 2008 View
European Union 2015 View
Malaysia 2012 View

Introduction Expand

The genetically engineered Carnation Moonberry (Event 25958) flowers are violet/mauve in color. This color is due to the biosynthesis of the anthocyanin pigment delphinidin, a pigment that is not produced in non-transgenic carnation. Three genes were introduced into the transgenic carnation: a functional dihydroflavonol reductase encoding gene (dfr) from petunia, a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), and a dihydroflavonol-4-reductase (dfr) construct from carnation in a dsDFR format. The dfr construct silences expression of the endogenous carnation dfr gene, allowing dominant expression of the introduced petunia dfr gene. 

Summary of Introduced Genetic Elements Expand

Code Name Type Promoter, other Terminator Copies Form
surB acetolactate synthase SM

CaMV 35S promoter and Cab 5’utr leader sequence

Acetohydroxy acid synthase gene terminator

F3’5’H flavonoid-3’,5’-hydroxylase PM

Snap dragon CHS promoter

Petunia D8 gene

dfr dihydroflavonol reductase PM

dfr promoter

dfr terminator

ds Carn DFR DFR gene-silencing construct PM

CaMV 35S promoter

CaMV 35S terminator


Characteristics of Dianthus caryophyllus (Carnation) Expand

Center of Origin Reproduction Toxins Allergenicity

The species is native to the Mediterranean region, including Europe, Asia, and northern Africa.

Carnation flowers are pollinated primarily by butterflies and sometimes by other insects.

The parts of the carnation plants are not thought to be toxic, and the flowers are considered edible.

Occupational exposure to carnation flowers has been associated with allergic reactions in humans.

Donor Organism Characteristics Expand

Latin Name Gene Pathogenicity
Nicotiana tabacum surB

Nicotiana tabacum is cultivated tobacco and it is well known for its health effects. Adverse health effects can result from human exposure via inhalation of tobacco smoke, or dermal or oral intake of tobacco products. 

Viola hortensis F3’5’H

Viola hortensis is a flowering plant with no human toxicity or allergenicity reported in the literature. There is a report on antithrombin effect caused by the extracts of Viola hortensis.

Petunia X hybrida dfr

Petunia X hybrid is a flowering plant. No toxicity to human or animals are reported. No allergy is linked to petunia exposure. In fact, petunia is considered to be among the safe flower plants to people who are allergic to flower pollens

Dianthus caryohpyllus ds Carn DFR

Carnation poisoning can be triggered by consumption of very high amounts of carnation leaves, which contain triterpenoid saponins. But carnation flowers are cut flowers and they are not supposed to be consumed orally. 

Modification Method Expand

Coming soon.

Characteristics of the Modification Expand

Coming soon.

Environmental Safety Considerations Expand

Coming soon.

Food and/or Feed Safety Considerations Expand

Coming soon.

Links to Further Information Expand

This record was last modified on Wednesday, December 21, 2016